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cryo-electron microscopy and tomography
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Midwest Center for Cryo-Electron Tomography (MCCET)

Resources and Equipment

Name for Equipment Description
Grid Storage Cryo-grid storage with puck holders
Leica ACE600 System Leica ACE600 System for glow discharge, carbon evaporation, and metal coating.
FEI Vitrobot FEI Vitrobot Mark IV cryo-plunger system
Leica Cryo-CLEM System Leica Cryo-CLEM System with cryo-CLEM stage and transfer shuttle, Leica DM6 FS automated wide-field upright microscope and motorized stage; filter systems for DAPI, GFP, Texas Red (mCherry), YFP, CFP, Cy3, and Cy5. Software for automation and image processing.
Leica DMi8 wide field fluorescence microscope Leica DMi8 widefield fluorescence microscope with Alveole PRIMO Micropatterning system.
Aquilos Cryo-FIB-SEM 2 Aquilos Cryo-FIB-SEM (with planned upgrade to Aquilos 2, July 2021) with dedicated cryo-stage cooling, a loading and transfer system, field emission SEM column.
Talos L120C 120kV Talos L120C with Ceta CMOS camera, room temperature specimen holders, Gatan 626 cryotransfer system and holders.
Talos Arctica 200kV Talos Arctica with Volta phase plates, STEM detector, Falcon 3C direct electron detector, Gatan BioQuantum Post-column Energy Filter with K3 direct electron detector, Ceta CMOS camera.
Krios G3i 300kV Titan Krios G3i with Volta phase plates, STEM detector, Falcon 3C direct electron detector, Gatan BioQuantum Post-column Energy Filter with K3 direct electron detector, Ceta CMOS camera.
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